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Image Search Results
Journal: Scientific Reports
Article Title: Rapid virus-free production of recombinant yellow fever virus envelope protein and its in-depth biophysical analysis
doi: 10.1038/s41598-025-33406-8
Figure Lengend Snippet: SEC-MALS of purified sE-17D and sE-Asibi proteins. 25 µL protein samples of a ) sE-17D (1 mg/mL) and b ) sE-Asibi (1 mg/mL) were loaded on an AdvanceBio SEC 300 Å HPLC column and separated at a flow rate of 0.25 mL/min with 150 mM sodium phosphate at pH 7.0. The UV trace recorded at 280 nm shows one major peak at 12 min elution time. MALS analysis of the peaks (bold lines) and calculation based on the first order Zimm Plot resulted in molecular weights of 58–59 kDa for sE-17D and sE-Asibi.
Article Snippet: The HPLC system was serially connected to an Agilent 1260 Infinity II DAD UV detector, a
Techniques: Purification